Retroviral vectors with foreign-sequence insertion between retroviral primer binding site and retroviral splice donor

A composition of matter comprising an isolated retroviral murine embryonic stem cell virus (MESV) vector comprising the following components obtained from a MESV, wherein said components are set forth in the following 5′ to 3′ order:

• a terminal sequence repeat (long terminal repeat, LTR);
• the retroviral primer binding site;
• the retroviral splice donor signal;
• the retroviral packaging signal;
• the retroviral splice acceptor signal;
• and
• a polypurine tract;
• wherein the vector further comprises a foreign sequence 1 located between the retroviral primer binding site and the retroviral splice donor, wherein foreign sequence 1 is selected from the group consisting of an enhancer-promoter sequence, an enhancer sequence, a cDNA sequence, a sequence for chromatin-modification, and a sequence for RNA processing signals, and further comprising a foreign sequence 2 inserted between the retroviral splice acceptor signal and the polypurine tract, wherein foreign sequence 2 is selected from the group consisting of a cDNA sequence, an enhancer sequence, a promoter sequence, a sequence for internal ribosomal entry sites (IRES), a sequence for chromatin-modification, and a sequence for RNA processing signals.

A composition of matter comprising an isolated retroviral murine embryonic stem cell virus (MESV) vector comprising the following components obtained from a MESV, wherein said components are set forth in the following 5′ to 3′ order:

• a terminal sequence repeat (long terminal repeat, LTR);
• the retroviral primer binding site;
• the retroviral splice donor signal;
• the retroviral packaging signal;
• an optional retroviral splice acceptor signal;
• and
• a polypurine tract (PP tract);
• wherein the vector further comprises a foreign sequence 1 located between the retroviral primer binding site and the retroviral splice donor, wherein foreign sequence 1 is selected from the group consisting of an enhancer-promoter sequence, an enhancer sequence, a cDNA sequence, a sequence for chromatin-modification, and a sequence for RNA processing signals, and
• wherein at least one terminal sequence repeat (LTR) contains a foreign sequence 3 selected from the group consisting of a cloning site for the deletion of the LTR-enhancer promoter, an enhancer sequence, a promoter sequence, a cDNA sequence, a sequence for chromatin-modification, and a sequence for RNA processing signals.