Oligosaccharide synthesizer
AssigneeHitachi High-Technologies Corporation
InventorsKisaburo Deguchi, Genzou Hirata, Masahito Ito, Hiroaki Nakagawa, Shinichiro Nishimura
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Abstract
After various sugar nucleotide solutions and glycosyltransferases (or primers) have been mixed, they are introduced into a reaction tank (column) with primers (or glycosyltransferases) immobilized thereon. Then solutions coming out of the reaction tank are led to an ultrafiltration column. The oligosaccharide synthesizer according to the present invention is equipped with a flow path for ensuring that glycosyltransferases or primers separated by the ultrafiltration column are returned into a container for storing each solution in a sample injector.
Claims7
A oligosaccharide synthesizer comprising:
- a container for storing buffer solution;
- a pump for feeding buffer solution;
- a sample injector comprising a container for storing a sugar nucleotide solution and a container for storing glycosyltransferase, said injector for mixing said sugar nucleotide solution and said glycosyltransferases and for injecting the mixture into a flow path for feeding said buffer solution;
- a reaction tank where a primer is immobilized, said tank used for reaction between solution injected out of said sample injector and said primer;
- an ultrafiltration column for separating said glycosyltransferase from sugar nucleotide and nucleotide;
- and
- a collection flow path for feeding said glycosyltransferase flowing out of said ultrafiltration column, into the container for storing glycosyltransferase of said sample injector.
A oligosaccharide synthesizer comprising:
- a container for storing buffer solution;
- a pump for feeding buffer solution;
- a sample injector further comprising:
- a container for storing a sugar nucleotide solution,
- a container for storing a primer, and
- a mixing tank for mixing the sugar nucleotide solution with said primer;
- wherein the solution mixed by said mixing tank being injected into the flow path for feeding said buffer solution by said sample injector;
- a reaction tank where said primer is immobilized, said tank being used for reaction between solution injected out of said sample injector and said primer;
- an ultrafiltration column for separating said primer from sugar nucleotide and nucleotide or oligosaccharide;
- a first flow path for feeding the primer coming out of the ultrafiltration column, into the primer container of said sample injector;
- and
- a second flow path for feeding the sugar nucleotide and nucleotide or oligosaccharide coming out of the ultrafiltration column, into a drain.
Dependencies are inferred from the claim text.