# US 7,070,983 B2 · Mutant Pseudomonas putida strains and methods of use thereof

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- Number: US 7,070,983 B2
- Document ID: US7070983B2
- Status: Granted
- Kind: B2
- Application number: 10/681,109
- Filed: 2003-10-09
- Published: 2005-04-28
- Granted: 2006-07-04
- Earliest claimed: 2003-10-09
- Assignees: National Food Research Institute
- Inventors: Kozo Ochi; Keiichi Hosokawa
- Web page: https://patentgrep.com/patent/US7070983B2

## CPC

- C12N 1/205: Bacterial isolates
- C12R 2001/40: Pseudomonas putida
- Y10S 435/877: Pseudomonas putida
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)
- C12R: INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- Y10S: TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS

## Abstract

The present invention relates to mutant strains of Pseudomonas putida and their use in the detoxification of industrial and/or toxic waste products.

## Claims

### Claim 1

An isolated Pseudomonas putida strain selected from the group consisting of FERM BP-08462, FERM BP-08463, FERM BP-08464, and FERM BP-08465.

### Claim 2 (depends on 1)

The isolated Pseudomonas putida strain of claim 1, which is FERM BP-08462.

### Claim 3 (depends on 1)

The isolated Pseudomonas putida strain of claim 1, which is FERM BP-08463.

### Claim 4 (depends on 1)

The isolated Pseudomonas putida strain of claim 1, which is FERM BP-08464.

### Claim 5 (depends on 1)

The isolated Pseudomonas putida strain of claim 1, which is FERM BP-08465.

### Claim 6 (depends on 2)

A composition comprising the isolated Pseudomonas putida strain of claim 2 and a carrier.

### Claim 7 (depends on 3)

A composition comprising the isolated Pseudomonas putida strain of claim 3 and a carrier.

### Claim 8 (depends on 4)

A composition comprising the isolated Pseudomonas putida strain of claim 4 and a carrier.

### Claim 9 (depends on 5)

A composition comprising the isolated Pseudomonas putida strain of claim 5 and a carrier.

### Claim 10

A composition comprising one or more isolated Pseudomonas putida strain(s) selected from the group consisting of FERM BP-08462, FERM BP-08463, FERM BP-08464, and FERM BP-08465; and a carrier.

### Claim 11 (depends on 2)

A method of detoxifying industrial wastes of toxic organic compounds, comprising contacting the industrial wastes with the Pseudomonas putida of claim 2 for a time to detoxify the industrial waste.

### Claim 12 (depends on 3)

A method of detoxifying industrial wastes of toxic organic compounds, comprising contacting the industrial wastes with the Pseudomonas putida of claim 3 for a time to detoxify the industrial waste.

### Claim 13 (depends on 4)

A method of detoxifying industrial wastes of toxic organic compounds, comprising contacting the industrial wastes with the Pseudomonas putida of claim 4 for a time to detoxify the industrial waste.

### Claim 14 (depends on 5)

A method of detoxifying industrial wastes of toxic organic compounds, comprising contacting the industrial wastes with the Pseudomonas putida of claim 5 for a time to detoxify the industrial waste.

### Claim 15 (depends on 10)

A method of detoxifying industrial wastes of toxic organic compounds, comprising contacting the industrial wastes with the composition of claim 10 for a time to detoxify the industrial waste.