Polynucleotide compositions encoding broad spectrum delta-endotoxins
AssigneeMonsanto Technology LLC
InventorsThomas Malvar, Komarlingam Sukavancaswaran Mohan, Sakuntala Sivasupramaniam
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Abstract
Disclosed are novel synthetically-modified B. thuringiensis chimeric crystal proteins having improved insecticidal activity and broader insect host range against coleopteran, dipteran and lepidopteran insects. Also disclosed are the nucleic acid segments encoding these novel peptides. Methods of making and using these genes and proteins are disclosed as well as methods for the recombinant expression, and transformation of suitable host cells. Transformed host cells and transgenic plants expressing the modified endotoxin are also aspects of the invention.
Claims14
An isolated polynucleotide encoding an insecticidal Bacillus thuringiensis hybrid protein comprising domains I and II of a first native B. thuringiensis delta-endotoxin Cry1A protein, domain III of a second native B. thuringiensis delta-endotoxin Cry1F protein, and all or a portion of a protoxin segment of a native delta endotoxin Cry1A, Cry1F or combination Cry1F-Cry1A protein.
A recombinant vector comprising a polynucleotide encoding an insecticidal Bacillus thuringiensis hybrid protein comprising domains I and II of a first native B. thuringiensis delta-endotoxin Cry1A protein, domain III of a second native B. thuringiensis delta-endotoxin Cry1F protein, and all or a portion of a protoxin segment of a native delta endotoxin Cry1A, Cry1F or combination Cry1F-Cry1A protein.
Dependencies are inferred from the claim text.