Midecamycin biosynthetic genes
AssigneeMeiji Seika Kaisha, Ltd.
InventorsNaoki Midoh, Shigeru Hoshiko, Takeshi Murakami
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Abstract
The present invention provides an isolated polypeptide comprising a nucleotide sequence encoding a protein which is involved in midecamycin biosynthesis, wherein the protein contains an amino acid sequence selected from SEQ ID NOs: 2 to 10, 13, 14, 16, 19, 20, 22 to 26, and 28 to 38 or a modified amino acid sequence of the amino acid sequence having one or more amino acid modifications without affecting activity of the protein.
Claims14
An isolated polynucleotide comprising a nucleotide sequence encoding a protein which is involved in midecamycin biosynthesis, wherein said protein comprises an amino acid sequence selected from the group consisting of the following sequences:
- (a) the amino acid sequence of SEQ ID NO:
- 3,
- (b) the amino acid sequence of ORF2 protein, which is encoded by a clone contained in the microorganism deposited under an accession number of FERM BP-8168, and
- (c) a modified amino acid sequence of (a) or (b) having one to 40 conservative amino acid modifications wherein said modified amino acid sequence has polyketide synthase activity.
An isolated polynucleotide comprising a nucleotide sequence encoding a functional domain of polyketide synthase (PKS) which is involved in midecamycin biosynthesis, wherein said domain comprises an amino acid sequence selected from the group consisting of the following sequences:
- (1) an amino acid sequence selected from amino acid residues 35–460, 577–929, 943–1169, 1457–1744, and 1759–1844 of SEQ ID NO:
- 3,
- (2) the amino acid sequence of a functional domain of ORF2 protein, which is encoded by a clone contained in the microorganism deposited under an accession number of FERM BP-8168 and
- (3) an amino acid sequence of either (1) or (2) having one to eight conservative amino acid modifications wherein said modified amino acid sequence has polyketide synthase activity.
An isolated polynucleotide comprising a nucleotide sequence encoding a protein which is involved in midecamycin biosynthesis, wherein said nucleotide sequence comprises a nucleotide sequence which can hybridize with a nucleotide sequence which encodes the amino acid sequence of SEQ ID NO:
- 3, under stringent conditions comprising 0.2×SSC in a 0.1% SDS solution at 60° C. for 15 minutes.
An isolated polynucleotide comprising a nucleotide sequence encoding a functional domain of polyketide synthase (PKS) which is involved in midecamycin biosynthesis, wherein said nucleotide sequence is a nucleotide sequence which can hybridize with a nucleotide sequence encoding an amino acid sequence selected from amino acid residues 35–460, 577–929, 943–1169, 1457–1744, and 1759–1844 of SEQ ID NO:
- 3, under stringent conditions
- wherein said stringent conditions comprise 0.2×SSC in a 0.1% SDS solution at 60° C. for 15 minutes.
Dependencies are inferred from the claim text.