US 7,070,960 B2Granted

Method of increasing product expression through solute stress

AssigneeChiron Corporation

InventorsBrian Maiorella, Duane Inlow, William Howarth

Life of this patent

Estimating the term…

Loading patent…

Abstract

A method of determining the optimal level of product expression and cell growth of animal cell culture is described. The method generally comprises culturing cells under conditions of solute stress, that is, under conditions whereby optimal cell growth or growth rate is decreased yet levels of product expression are increased. In a preferred embodiment of the invention is described a method of increasing the yield of monoclonal antibodies comprising culturing hybridoma cells in an environment of solute stress. One approach to the creation of such an environment is the addition of inorganic salts, organic polyols, or metabolic products to the culture medium. One- to three-fold increases in antibody yield have been obtained by these methods.

Claims17

Dependent claims

A method of optimizing the condition for product expression in animal cell culture wherein the concentration of a solute of interest in a culture medium composition for optimal product expression is different from the concentration of said solute in the culture medium composition determined for optimal cell growth, which method comprises:

a) growing the animal cell culture in a culture medium to determine the condition for optimal cell growth;
b) varying the concentration of the solute in the culture medium to a concentration above that optimal for cell growth, which concentration is effective to create an environment of solute stress on the cell culture as expressed by an inhibitory effect on cell growth or cell density of said cell culture while
monitoring the product expression as concentration of the solute is varied in the culture medium to detennine optimal product expression;
and
c) selecting the solute concentration that provides the optimal combination of cell growth and product expression, wherein said solute is selected from the group consisting of an inorganic salt or ion thereof, an organic polyol, and a metabolite.

2 more elements

Dependencies are inferred from the claim text.

Facts

Application: 11/124,746Filed 9 May 2005 · continuation
Priority: 18 Nov 1987
Published: US 2005/0221393 A16 Oct 2005
Granted: 4 Jul 2006
Term ends: Not estimatedThe source records don’t support an estimate.

Classification5 codes

C12N 5/163
Animal cells: one of the fusion partners being a B or a T lymphocyte
C12N 2500/12
Metals; Metal chelators: light metals, i.e. alkali, alkaline earth, Be, Al, Mg
C12N 2500/50
Specific components of cell culture medium: soluble polymers, e.g. polyethyleneglycol [PEG]
C12N 2500/90
Specific components of cell culture medium: serum-free medium, which may still contain naturally-sourced components

Family

Application07/122,015Parent of this continuation-in-part · filed 18 Nov 1987
Application07/443,445Parent of this continuation · filed 29 Nov 1989
Application07/841,906Parent of this continuation · filed 26 Feb 1992
Application08/482,421Parent of this continuation · filed 7 Jun 1995
Application09/867,948Parent of this continuation · filed 30 May 2001
US 2005/0221393 A1Pre-grant publication · published 6 Oct 2005
US 6,905,847 B2Parent of this continuation
US 6,238,891 B1Parent of this continuation
Application07/443,445Parent of this continuation-in-part
US 2002/0119530 A1Published application
US 7,070,960 B2This patent

From the US filing history. Foreign family members aren’t listed.