Method for assaying glycated protein
AssigneeKikkoman Corporation
InventorsRyoichi Sakaue, Ayumi Arai, Naoki Kajiyama, Yasuji Koyama
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Abstract
Based on a principle that is different to that of a conventional enzymatic method, the present invention provides a novel method for assaying a glycated protein by a simple procedure, within a short period of time, and with high accuracy, and a reagent kit for assaying used in the method. The method for assaying a glycated protein in a sample is realized by treating a glycated protein-containing sample with protease to liberate a glycated peptide, preferably an α-glycated peptide, particularly preferably an α-glycated dipeptide, from a glycated protein, allowing an oxidase to react with the liberated glycated peptide, and determining the produced hydrogen peroxide.
Claims28
A method for assaying the presence and/or amount of an alpha glycated peptide in a sample comprising:
- treating a sample suspected of containing an α-glycated peptide with at least one oxidase which produces hydrogen peroxide upon reacting with the α-glycated peptide, and
- determining the presence and/or amount of a generated product or consumed substance by said reaction.
A method for assaying the presence and/or amount of a glycated protein in a sample comprising:
- treating a sample suspected of containing a glycated protein with at least one protease and
- determining the presence or absence of, and/or amount of, fructosyl valyl histidine liberated from said sample.
A kit comprising:
- an oxidase which produces hydrogen peroxide by reacting with an α-glycated peptide.
Dependencies are inferred from the claim text.