Loading patent…
AssigneeBecton, Dickinson and Company
InventorsZbigniew Darzynkiewicz, Frank Traganos, Gloria Juan, Stefan Gruenwald
Estimating the term…
Methods, reagents, and kits are provided that permit flow cytometric determination of the phosphorylation status of retinoblastoma susceptibility gene protein (pRB) in individual cells. Methods are described that permit the hypophosphorylated, active, form of pRB to be measured either as an absolute quantity or as a proportion of total cellular pRB. Further described are methods that permit pRB phosphorylation status to be correlated with cell cycle phase and with protein components of the cell cycle. Screening of chemical compounds for antiproliferative and antineoplastic activity using the flow cytometric assays is demonstrated. Reagent kits that facilitate the subject methods are also provided.
A kit for detecting the phosphorylation status of pRB in individual cells, comprising:
A kit for determining the relative intracellular conformational states of a protein, wherein said conformational states result from the phosphorylation state of said protein, comprising:
A kit for determining the relative intracellular phosphorylation states of a protein, comprising:
A kit for determining the relative intracellular phosphorylation states of a protein, comprising:
Dependencies are inferred from the claim text.