Surface treatment
AssigneeInternational Business Machines Corporation
InventorsSergey Amontov, Bruno Michel, Sally Ann Swanson, Heiko Wolf
Life of this patent
Estimating the term…
Abstract
Described is a process for producing a biomolecular monolayer on a biosensor surface comprising the steps of: reacting a biosensor surface with a solution of heterobifunctional reagent having a first functional group and a second functional group, the first functional group being capable of forming a covalent bond to the biosensor surface groups, the second functional group forming a covalent bond with a homobifunctional polymer to obtain a self-assembled monolayer, and thereafter reacting the monolayer with capture molecules.
Claims2
A process for producing a biomolecular monolayer on a surface, comprising:
- reacting homobifunctional polyethylene glycol (PEG) N-hydroxy succinimide (NHS) with aminopropyl trimethoxysilane at between 46 and 50 degrees Celsius for between 30 and 60 minutes to form a NHS-PEG-triethoxysilane reagent, wherein the aminopropyl trimethoxysilane comprises a first functional group for forming a covalent bond to a surface group and a second functional group for forming a covalent bond with the homobifunctional PEG NHS;
- exposing the surface to the NHS-PEG-triethoxysilane reagent to form the covalent bond between the surface and first functional groups and obtain a self-assembled monolayer;
- and thereafter
- reacting the monolayer with biomolecules.
Dependencies are inferred from the claim text.