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US 7,070,921 B2Granted
Molecular modification assays
AssigneeMolecular Devices, LLC
InventorsWei Huang, Merl F. Hoekstra, J. Richard Sportsman, Ewald A. Terpetschnig
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Abstract
Assays for detecting molecular modifications such as phosphate modifications and the presence and/or activity of enzymes and other agents involved in facilitating or otherwise regulating such modifications.
Claims21
A method of detecting the activity of an enzyme that performs a phosphate modification on a substrate to form a product in a sample, comprising:
- contacting the substrate with the enzyme in the sample;
- contacting the sample with a binding partner that specifically binds to the substrate or to the product, but not to both, wherein the binding partner includes Ga(III) ion that is required for binding between the binding partner and the substrate or the product;
- detecting a response, based on luminescence polarization, indicative of the extent of binding between the substrate or the product and the binding partner without separating the bound substrate or product from the unbound substrate or product;
- and correlating the response with the activity of the enzyme.
1 more element
Dependencies are inferred from the claim text.