Method for enhancement of sperm survival using soluble peripheral protein fraction of APM
OwnerWilliam Vincent Holt, Alireza Fazeli +1inventors
InventorsWilliam Vincent Holt, Alireza Fazeli, Paul Frederick Watson
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Abstract
A method of improving and/or prolonging sperm viability which comprises contacting spermatozoa with an isolated, cell-free protein fraction of oviductal apical plasma membrane (APM). This finds use in maintenance of sperm viability to increase the success rate of artificial insemination (AI).
Claims10
A method of improving and/or prolonging sperm viability which comprises contacting spermatozoa with an isolated, cell-free, salt-extracted, soluble peripheral protein fraction of oviductal apical plasma membrane.
A method of improving and/or prolonging sperm viability following cryopreservation which comprises contacting spermatozoa with an isolated, cell-free, salt-extracted, soluble peripheral membrane protein fraction of oviductal apical plasma membrane.
A method of improving and/or prolonging sperm viability during cryopreservation which comprises contacting spermatozoa with an isolated, cell-free, salt-extracted, soluble peripheral membrane protein fraction of oviductal apical plasma membrane.
A method of improving and/or prolonging sperm viability during in vitro fertilisation which comprises contacting spermatozoa with an isolated, cell-free, salt-extracted, soluble perpheral membrane protein fraction of oviductal apical plasma membrane.
A method for improving and/or prolonging sperm viability which comprises contacting spermatozoa with an isolated, cell-free, salt-extracted, soluble peripheral membrane protein fraction of oviductal apical plasma membrane in which the concentration of the protein fraction is between approximately 0.1 μg/L and approximately 1 g/L.
A method of improving and/or prolonging semen survival following sex-sorting of the spermatozoa for X- (female) and Y-bearing (male) spermatozoa cells which comprises contacting spermatozoa with an isolated, cell-free, salt-extracted, soluble peripheral protein fraction of oviductal apical plasma membrane.
Dependencies are inferred from the claim text.