Back to results
US 7,070,789 B2Granted
Recombinant Newcastle disease virus nucleoprotein mutant as a marker vaccine
AssigneeAkzo Nobel N.V.
InventorsTeshome Mebatsion, Marcus Josephus Marie Koolen
Life of this patent
Estimating the term…
Abstract
The present invention provides a NDV mutant that is suited as a marker vaccine strain. The NDV mutant is not able to express an immunodominant epitope of the nucleoprotein (NP).
Claims20
A viable Newcastle disease virus (NDV) mutant lacking an immunodominant epitope found on an unmodified NDV protein as a result of a mutation in a gene encoding the protein, said epitope that is lacking being located within a region of the nucleoprotein (NP) of an unmodified NDV and having the amino acid sequence (447–455) shown in SEQ ID No. 1.
A method for detecting NDV infection in poultry comprising the steps of:
- (i) incubating a sample suspected of containing anti-NDV antibodies;
- with a fragment of the NP region of NDV comprising an amino acid sequence selected from the group consisting of sequence (447–455) and sequence (443–460) as shown in SEQ. ID No. 2 as the sole epitope,
- (ii) allowing the formation of antibody-antigen complexes, and
- (iii) detecting the presence of the antibody-antigen complexes.
1 more element
Dependencies are inferred from the claim text.