Loading patent…
AssigneeThe Mathilda and Terence Kennedy Institute of Rheumatology
InventorsYuti Chernajovsky, Richard Neve, Marc Feldmann
Estimating the term…
The present invention relates to a receptor molecule which binds to TNF comprising all or a functional portion of the extracellular domain (ECD) of two or more TNF-Rs linked via one or more polypeptide linkers. The receptor can further comprise a signal peptide of a secreted protein, such as the signal peptide of the extracellular domain of the TNF-R or the signal peptide of a cytokine. The invention also relates to isolated DNA encoding a receptor molecule which binds to TNF, comprising two or more sequences encoding all or a functional portion of the ECD of TNF-Rs linked via one or more sequences encoding a polypeptide linker. The invention further relates to a method of making a construct which expresses all or a functional portion of the ECD of two or more TNF-Rs linked via one or more polypeptide linkers and cells which express the construct. The invention also relates to a method of inhibiting the biological activity of TNF in a host comprising administering to the host an effective amount of a receptor molecule of the present invention. The invention further relates to receptor molecules which bind cytokines that bind to receptor molecules comprising more than one subunit.
An isolated DNA encoding a receptor molecule which binds to tumor necrosis factor comprising two extracellular domains of tumor necrosis factor receptors linked to a polypeptide linker, wherein said polypeptide linker is covalently bonded to said extracellular domains via peptide bonds, wherein the receptor molecule is capable of binding to a tumor necrosis factor trimer in a stoichiometric ratio of almost 1:1, and wherein the DNA comprises the nucleic acid sequence set forth in SEQ ID NO:1.
An isolated DNA encoding a receptor molecule which binds to tumor necrosis factor comprising two extracellular domains of tumor necrosis factor receptors linked to a polypeptide linker, wherein said polypeptide linker is covalently bonded to said extracellular domains via peptide bonds, wherein the receptor molecule is capable of binding to a tumor necrosis factor trimer in a stoichiometric ratio of almost 1:1, and wherein the polypeptide comprises consecutive amino acids having the amino acid sequence set forth in SEQ ID NO:2.
A receptor molecule which binds to tumor necrosis factor comprising two extracellular domains of tumor necrosis factor receptors linked to a polypeptide linker, wherein the molecule comprises the amino acid sequence of SEQ ID NO:2.
A method of making a construct which expresses extracellular domains of two tumor necrosis factor receptors linked to a polypeptide linker, comprising the steps of:
An isolated cell which expresses a receptor molecule encoded by the DNA having a nucleic acid sequence comprising the nucleic acid sequence set forth in SEQ ID NO:1.
Dependencies are inferred from the claim text.