Polypeptides containing polymorphisms of the repeated regions of pertactin in bordetella pertussis, bordetella parapertussis, and bordetella bronchiseptica, their use in diagnostics, and in immunogenic compositions

An immunogenic composition comprising a mixture of purified pertactins or pertactin fragments comprising Region II,

• wherein said pertactins or pertactin fragments are of Bordetella bronchiseptica, Bordetella parapertussis, or Bordetella pertussis,
• in an amount sufficient to induce a humoral or cellular immune response in an animal to which the immunogenic composition is administered, and
• wherein the number of PQP amino acid sequences in Region II differs between at least two of said purified pertactins or pertactin fragments.

An immunogenic composition comprising a purified Bordetella bronchiseptica pertactin or pertactin fragment comprising Region II,

• in an amount sufficient to induce a humoral or cellular immune response in an animal to which the immunogenic composition is administered,
• wherein the number of PQP amino acid sequences in Region II of said Bordetella bronchiseptica pertactin or pertactin fragment is 6, 8, or 9.

An immunogenic composition comprising a purified Bordetella bronchiseptica pertactin or pertactin fragment comprising region I,

• in an amount sufficient to induce a humoral or cellular immune response in an animal to which the immunogenic composition is administered,
• wherein the number of GGXXP (SEQ ID NO:
• 25) amino acid sequences in Region I of said Bordetella bronchiseptica pertactin or pertactin fragment is 1 or 2.

An immunogenic composition comprising a purified Bordetella bronchiseptica pertactin or pertactin fragment comprising Region I,

• in an amount sufficient to induce a humoral or cellular immune response in an animal to which the immunogenic composition is administered,
• wherein the number of GGAVP (amino acids 13 to 17 of SEQ ID NO:
• 8) amino acid sequences in Region I of said Bordetella bronchiseptica pertactin or pertactin fragment is 1 or 3.

An immunogenic composition comprising a purified Bordetella bronchiseptica pertactin or pertactin fragment comprising Region I,

• in an amount sufficient to induce a humoral or cellular immune response in an animal to which the immunogenic composition is administered,
• wherein the number of GGFGP (amino acids 23-27 of SEQ ID NO:
• 8) amino acid sequences in Region I of said Bordetella bronchiseptica pertactin or pertactin fragment is 2.

An immunogenic composition comprising a purified Bordetella bronchiseptica pertactin or pertactin fragment comprising Region I,

• in an amount sufficient to induce a humoral or cellular immune response in an animal to which the immunogenic composition is administered,
• wherein Region I of said Bordetella bronchiseptica pertactin or pertactin fragment comprises the amino acid sequence GGFDP (amino acids 23-27 of SEQ ID NO:
• 9).

An immunogenic composition comprising a purified Bordetella bronchiseptica pertactin or pertactin fragment comprising Region I,

• in an amount sufficient to induce a humoral or cellular immune response in an animal to which the immunogenic composition is administered,
• wherein Region I of said Bordetella bronchiseptica pertactin or pertactin fragment comprises the amino acid sequence GGGVP (amino acids 13-17 of SEQ ID NO:
• 9).