Back to results
US 7,069,151 B2Granted
Mapping of differential display of proteins
AssigneeThe Regents of the University of Michigan
InventorsDavid M. Lubman, Bathsheba E. Chong, Stephen J. Parus
Life of this patent
Estimating the term…
Abstract
The present invention relates to protein separation systems and methods capable of resolving and characterizing large numbers of cellular proteins. In particular, the present invention provides a novel mass mapping system and methods for the differential display of proteins. The present invention further provides novel methods for displaying differential protein expression between two samples. In particular, the present invention provides novel method of mapping differential expression of proteins in non-cancerous, pre-cancerous, and cancerous cells.
Claims35
A method of producing protein profile maps, comprising:
- a) providing:
- i) a first sample comprising a plurality of proteins;
- ii) a second sample comprising a plurality of proteins;
- iii) a separating apparatus, wherein said separating apparatus separates proteins based on a physical property;
- iv) a mass spectroscopy apparatus;
- and
- b) treating said first and second samples with said separating apparatus to produce a first separated protein sample and a second separated protein sample, wherein said first and second separated protein samples are collected from said separating apparatus in a plurality of fractions, each of said fractions defined by a physical property;
- and
- c) analyzing said plurality of fractions from each of said first and second separated protein samples with said mass spectroscopy apparatus to produce a protein profile map for each of said first and second samples, wherein said protein profile maps display protein abundance and mass of said first protein sample and said second protein sample, and wherein said protein profile maps displays each protein as a separate band corresponding to said mass of said first protein sample and said second protein sample, and wherein the intensity of said band corresponds to said protein abundance of said first protein sample and said second protein sample;
- and wherein said protein profile maps for each of said first and second samples are displayed side by side.
7 more elements
A system for the production of a data representation of a protein profile map, comprising:
- a) a non-porous reverse phase HPLC separating apparatus;
- b) an automated sample handling apparatus configured to receive first and second separated protein samples from said reverse phase HPLC separating apparatus;
- c) a mass spectroscopy apparatus configured to receive proteins from said automated sample handling apparatus;
- d) a processor configured to produce a data representation of a protein profile map for said first and second separated protein samples analyzed by said mass spectroscopy apparatus, wherein said protein profile map displays protein abundance and mass of a separated protein sample, wherein said protein profile map displays proteins as separate bands corresponding to said protein abundance and mass of said separated protein sample, and wherein the intensity of said bands corresponds to the abundance of said proteins, wherein said protein profile maps for each of said first and second samples are displayed side by side;
- and
- e) a display apparatus that displays said protein profile maps.
3 more elements
A method of producing protein profile maps, comprising:
- a) providing:
- i) a first sample comprising a plurality of proteins;
- ii) a second sample comprising a plurality of proteins;
- iii) a separating apparatus, wherein said separating apparatus separates proteins based on a physical property;
- iv) a mass spectroscopy apparatus;
- and
- b) treating said first and second samples with said separating apparatus to produce a first separated protein sample and a second separated protein sample, wherein said first and second separated protein samples are collected from said separating apparatus in a plurality of fractions, each of said fractions defined by a physical property;
- c) analyzing said plurality of fractions from each of said first and second separated protein samples with said mass spectroscopy apparatus to produce first and second protein profile maps for each of said first and second protein samples, wherein said protein profile maps display protein abundance and mass of said first protein sample and said second protein sample;
- and
- d) displaying a differential display protein map of said first and second protein profile maps, wherein said differential display protein map displays the difference in protein abundance versus mass between proteins in said first and second protein samples, and wherein said differential display protein profile map displays the difference in protein abundance between each protein as a separate band corresponding to said mass of said first protein sample and said second protein sample, and wherein the intensity of said band corresponds to the difference in protein abundance.
7 more elements
Dependencies are inferred from the claim text.