Commercial production of recombinant manganese-dependent peroxidase in plants
AssigneesProdi Gene, Inc., Geneucor International, Inc.
InventorsElizabeth Hood, John Howard, Richard Clough, Kameshwari Pappu
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Abstract
Expression of a recombinant manganese-dependent peroxidase in plants is described. Methods for the recovery of transgenic corn seeds that express high levels of functional manganese-dependent peroxidase are also provided. Manganese-dependent peroxidase vectors are engineered to contain sequences directing seed-preferred expression as well as sequences for cell wall-targeted localization.
Claims23
A viable corn plant expressing manganese peroxidase, wherein the manganese peroxidase is preferentially directed to the cell wall of the plant.
A Plant cells of a corn plant expressing manganese peroxidase such that the manganese peroxidase is preferentially directed to the cell wall and the plant cells is viable.
A method for expressing manganese peroxidase in a viable corn plant comprising expressing in a corn plant cell a nucleic acid construct comprising a sequence encoding a manganese peroxidase protein, wherein the manganese peroxidase is preferentially directed to the cell wall.
A method for producing increased levels of manganese peroxidase in a corn plants comprising:
- (i) expressing in a corn plant tissue a first nucleotide sequence encoding a manganese peroxidase protein and a second nucleotide sequence operably linked to the first nucleotide sequence and which preferentially directs expression of the first nucleotide sequence to the cell wall of the plant tissue;
- (ii) culturing a plant from the plant tissue;
- (iii) growing and harvesting the plant such that manganese peroxidase is expressed.
A method of producing manganese peroxidase at levels of at least about 0.1% total soluble protein, comprising providing biomass from a plurality of corn plants, of which at least certain plants contain a nucleotide molecule comprising a heterologous nucleotide sequence coding for the manganese peroxidase, wherein the nucleotide sequence is operably linked to a nucleotide sequence preferentially directing of the manganese peroxidase to the cell wall of the certain plants to produce manganese peroxidase expressed at levels of at least about 0.1% total soluble protein.
Dependencies are inferred from the claim text.