Nucleic acid sequences and methods of use for the production of plants with modified polyunsaturated fatty acids
AssigneeMonsanto Technology LLC
InventorJoAnne J. Fillatti
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Abstract
The present invention is directed to nucleic acid molecules and nucleic acid constructs, and other agents associated with fatty acid synthesis, particularly the ratios of saturated and unsaturated fats. Moreover, the present invention is directed to plants incorporating such agents where the plants exhibit altered ratios of saturated and unsaturated fats. In particular, the present invention is directed to plants incorporating such agents where the plants exhibit altered ratios of monounsaturated to polyunsaturated fatty acids.
Claims13
A transformed soybean plant having a nucleic acid molecule that comprises
- (a) a first promoter operably linked to a first nucleic acid molecule having a first nucleic acid sequence that has 90% or greater identity to SEQ ID NO:
- 1, its complement and fragments of either, wherein said first nucleic acid molecule is capable of suppressing endogenous expression of Fad2-1, and
- (b) a second nucleic acid molecule with a second nucleic acid sequence that has 90% or greater identity to SEQ ID NO:
- 14, its complement and fragments of either, wherein the second nucleic acid molecule is operable linked to the first promoter or a second promoter, wherein said first nucleic acid molecule is capable of suppressing endogenous expression of Fad3-1.
A method of producing a soybean plant having a seed with reduced linolenic acid content comprising:
- transforming a soybean plant with a nucleic acid molecule that comprises
- (a) a first promoter operably linked to a first nucleic acid molecule having a first nucleic acid sequence that has 90% or greater identity to SEQ ID NO:
- 1 and its complement, wherein said first nucleic acid molecule is capable of suppressing endogenous expression of Fad2-1,and
- (b) a second nucleic acid molecule having a second nucleic acid sequence that has 90% or greater identity to SEQ ID NO:
- 14 and its complement, wherein the second nucleic. acid molecule is operably linked to the first promoter or a second promoter and capable of suppressing endogenous expression of Fad3-1;
- and
- growing said plant, wherein said plant produces seed with less linolenic acid than a plant having a similar genetic background but lacking said nucleic acid molecule.
A method of producing a soybean plant having a seed with increased oleic acid content comprising:
- transforming a soybean plant with a nucleic acid molecule that comprises
- (a) a first promoter operably linked to a first nucleic acid molecule having a first nucleic acid sequence that has 90% or greater identity to SEQ ID NO:
- 1 and complement thereof, wherein said first nucleic acid molecule is capable of suppressing endogenous expression of Fad2-1, and
- (b) a second nucleic acid molecule having a second nucleic acid sequence that has 90% or greater identity to SEQ ID NO:
- 14 and complements thereof, wherein the second nucleic acid molecule is operably linked to the first promoter or a second promoter and capable of suppressing endogenous expression of Fad3-1;
- and growing said plant, wherein said plant produces seed with more oleic acid than a plant having similar genetic background but lacking said nucleic acid molecule.
Dependencies are inferred from the claim text.