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US 7,067,719 B1Granted
Transformation of Allium sp. with Agrobacterium using embryogenic callus cultures
AssigneeSeminis Vegetable Seeds, Inc.
InventorJohn Reynolds
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Abstract
The present invention relates to a method for transforming Allium species with a heterologous gene using Agrobacterium.
Claims13
A method for transforming a plant or plant tissue of an Allium cepa or Allium fistulosum with a DNA of interest from a heterologous gene, the method comprising the steps of:
- contacting an embryogenic callus from a plant of an Allium cepa or Allium fistulosum with a bacterium belonging to the genus Agrobacterium which contains a DNA of interest from a heterologous gene and obtaining a transformed Allium cepa or Allium fistulosum embryogenic callus under selective conditions.
A method for transforming a plant or plant tissue of an Allium cepa or Allium fistulosum with a DNA of interest from a heterologous gene, the method comprising the steps of:
- a. culturing immature embryos or flower buds from a plant of an Allium cepa or Allium fistulosum on an initiation medium for a period of from 2 to 6 months until an embryogenic callus forms on the embryos or flower buds:
- b. transferring the embryonic callus to a coculture medium and contacting the embryogenic callus with a suspension of Agrobacterium rhizogenes or Agrobacterium tumefaciens containing a DNA of interest from a heterologous gene;
- and
- c. obtaining a transformed Allium cepa or Allium fistulosum embryogenic callus under selective conditions.
1 more element
Dependencies are inferred from the claim text.