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US 7,067,716 B2Granted
Methods and compositions for the introduction of molecules into cells
AssigneeStine Biotechnology
InventorsBruce Marvin Held, Herbert Martin Wilson, Liming Hou, Carol Jean Lewnau, Janelle Christine Eby
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Abstract
The present invention is directed to the introduction of molecules, including nucleic acids, carbohydrates, plant growth regulators and peptides into cells and tissues. The present invention is also directed to media and methods for enhancing embryogenic callus production of elite lines of soybean.
Claims14
A method for the introduction of one or more peptides into a cell, wherein the method comprises:
- (a) preparing a solution containing said peptides;
- (b) supplying said solution for conversion to aerosol droplets which are produced by a microflow nebulizer at a flow rate of between about 1 μl/minute to about 100 μl/minute;
- (c) producing said aerosol droplets comprising said peptides;
- (d) accelerating said aerosol droplets toward said cell;
- (e) impacting said cell with said accelerated aerosol droplets;
- and
- (f) producing a cell containing said peptides, wherein the cell is plant cell.
4 more elements
Dependencies are inferred from the claim text.