Ribozymes targeting bradeion transcripts and use thereof
AssigneeNational Institute of Advanced Industrial Science and Technology
InventorManami Tanaka
Life of this patent
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Abstract
This invention relates to: a set of ribozymes which comprises at least one ribozyme capable of binding and cleaving Bradeion α mRNA which corresponds to DNA comprising a nucleotide sequence shown in SEQ ID NO:1, and at least one ribozyme capable of binding to and cleaving Bradeion β mRNA which corresponds to DNA comprising a nucleotide sequence shown in SEQ ID NO:3; a set of vectors comprising ribozymes; a pharmaceutical composition comprising set of vectors; and a method for treating a subject with a cancer using set of vectors.
Claims12
A set of ribozymes which comprises at least one ribozyme capable of binding and cleaving Bradeion α mRNA which corresponds to DNA comprising a nucleotide sequence shown in SEQ ID NO:1, and at least one ribozyme capable of binding to and cleaving Bradeion β mRNA which corresponds to DNA comprising a nucleotide sequence shown in SEQ ID NO:3, wherein said ribozyme capable of binding to and cleaving Bradeion α mRNA targets a sequence selected from the group consisting of RNA sequences corresponding to nucleotide numbers 569-583, 588-602, 595609 and 699-713 of SEQ ID NO:1, and said ribozyme capable of binding and cleaving Bradeion β mRNA targets a sequence selected from the group consisting of RNA sequences corresponding to nucleotide numbers 108-122, 157-171, 304-318 and 378-392 of SEQ ID NO:3.
A set of ribozymes which comprises at least one ribozyme capable of binding and cleaving Bradeion α mRNA which corresponds to DNA comprising a nucleotide sequence shown in SEQ ID NO:1, and at least one ribozyme capable of binding to and cleaving Bradeion β mRNA which corresponds to DNA comprising a nucleotide sequence shown in SEQ ID NO:3, wherein said ribozymes are a combination of at least one ribozyme selected from the group consisting of ribozymes of SEQ ID NO:5, SEQ ID NO:6, SEQ ID NO:7 and SEQ ID NO:8, and at least one ribozyme selected from the group consisting of ribozymes of SEQ ID NO:9, SEQ ID NO:10, SEQ ID NO:11 and SEQ ID NO:12 and a heterodimeric ribozyme that is composed of an RNA sequence of SEQ ID NO:13 and an RNA sequence of SEQ ID NO:14.
Dependencies are inferred from the claim text.