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US 7,067,644 B2Granted
Double transposition methods for manipulating nucleic acids
AssigneeWisconsin Alumni Research Foundation
InventorsIgor Yu Goryshin, Todd A Naumann, William S Reznikoff
Life of this patent
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Abstract
Methods are provided for manipulating nucleic acid to produce gene fusions, to delete or clone a portion of a chromosome, or to insert a sequence into a chromosome. The methods employ sequential transposition processes using two or more pairs of inverted repeat transposase-interacting sequences on a transposable polynucleotide wherein each pair of transposase-interacting sequences interacts with a distinct transposase enzyme.
Claims8
A polynucleotide comprising:
- distinct first and second transposase-interacting repeat sequence pairs, each sequence pair having a first member and a second member provided in an inverted orientation relative to the first member, each sequence pair having a specificity for binding to and interacting with a distinct transposase enzyme, the first sequence pair flanking the second sequence pair;
- and
- a first sequence for conferring selectability upon a host cell, wherein the selectability conferring sequence is flanked by the first member of the first sequence pair and by the first member of the second sequence pair.
Dependencies are inferred from the claim text.