Method to provide bacterial ghosts provided with antigens
AssigneeApplied NanoSystems B.V.
InventorsCornelis J. Leenhouts, Ranjan Ramasamy, Anton Steen, Jan Kok, Girbe Buist, Oscar P. Kuipers
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Abstract
Methods for improving binding of a proteinaceous substance to cell-wall material of a Gram-positive bacterium are disclosed. The proteinaceous substance includes an AcmA cell-wall binding domain, homolog or functional derivative thereof. The method includes treating the cell-wall material with a solution capable of removing a cell-wall component such as a protein, lipoteichoic acid or carbohydrate from the cell-wall material and contacting the proteinaceous substance with the cell-wall material.
Claims19
A method for binding a proteinaceous substance to cell-wall material of a Gram-positive bacterium, said method comprising:
- treating said cell-wall material with an acid solution, thus removing a cell-wall component selected from the group consisting of a protein, a lipoteichoic acid, a carbohydrate, and combinations thereof from said cell-wall material, thus producing spherical peptidoglycan microparticles;
- and
- contacting said spherical peptidoglycan microparticles with a proteinaceous substance, thus binding said proteinaceous substance to said spherical peptidoglycan microparticles;
- wherein said proteinaceous substance comprises an immunogenic determinant and an AcmA cell-wall binding domain, homolog, or functional derivative thereof that binds to the treated cell-wall material.
A method for binding a proteinaceous substance to cell-wall material of a Gram-positive bacterium, said proteinaceous substance comprising an AcmA cell wall binding domain, homolog, or functional derivative thereof capable of binding to said cell-wall material of the Gram-positive bacterium, said method comprising:
- treating said cell-wall material with an acid solution, thus removing a cell-wall component selected from the group consisting of a protein, a lipoteichoic acid, a carbohydrate, and combinations thereof from said cell-wall material, thus producing spherical peptidoglycan microparticles;
- and
- contacting said spherical peptidoglycan microparticles with said proteinaceous substance, so as to bind said proteinaceous substance to said spherical peptidoglycan microparticles.
A method for binding a proteinaceous substance to cell-wall material of a Gram-positive bacterium, said method comprising:
- treating the Gram-positive bacterium with an acid solution;
- and
- contacting the acid treated Gram-positive bacterium with a heterologous protein and means for attaching the heterologous polypeptide to the acid treated Gram-positive bacterium.
Dependencies are inferred from the claim text.