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US 7,067,631 B2Granted
Fused DNA sequence, fused protein expressed from said fused DNA sequence and method for expressing said fused protein
AssigneeFujirebio Inc. (Fujirebio Kabushiki Kaisha)
InventorsEiichi Ueno, Noboyuki Fujii, Masahisa Okada
Life of this patent
Estimating the term…
Abstract
Disclosed are a fused DNA sequence which comprises a DNA sequence of a heat-resistant protein, fused directly or indirectly to a DNA sequence coding a selected protein or peptide, a fused protein expressed from the fused DNA sequence, and a method for expressing the fused protein.
Claims5
A fused protein expressed from a fused DNA sequence which comprises a first DNA sequence encoding a heat-resistant ferredoxin or a heat-resistant adenylate kinase fused directly or indirectly to the 5′ end of a second DNA sequence encoding a selected protein or peptide in a reading frame, so that
- (1) the fused DNA sequence produces a protein in which the heat-resistant ferredoxin or the heat-resistant adenylate kinase is attached to the amino terminus of the selected protein or peptide when expressed in a host cell, and
- (2) the fused DNA is expressed in the host cell at a higher level as compared to a corresponding DNA which comprises the DNA sequence encoding a selected protein or peptide but does not comprise the DNA sequence encoding a heat-resistant ferredoxin or a heat-resistant adenylate kinase.
Dependencies are inferred from the claim text.