Antimicrobial proteins
AssigneesCommonwealth Scientific and Industrial Research Organisation, The State of Queensland, Department of Primary Industries, The University of Queensland, Bureau of Sugar Experiment Stations, Queensland University of Technology
InventorsJohn Michael Manners, John Paul Marcus, Kenneth Clifford Goulter, Jodie Lyn Green, Neil Ivan Bower
Life of this patent
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Abstract
A new family of antimicrobial proteins is described. Prototype proteins can be isolated from Macadamia integrifolia as well as other plant species. DNA encoding the protein is also described as well as DNA constructs which can be used to express the antimicrobial protein or to introduce the antimicrobial protein into a plant. Compositions comprising the antimicrobial protein or the antimicrobial protein per se can be administered to plants or mammalian animals to combat microbial infestation.
Claims23
An isolated or purified protein fragment having antimicrobial activity, wherein said protein fragment is a polypeptide consisting of the sequence, C-3X-C-nX-C-3X-C (SEQ ID NOS:
- 38 and 39), wherein n is 11 or 12, X is any amino acid residue other than cysteine, and C is cysteine.
An isolated or purified protein having antimicrobial activity and having a sequence selected from the group consisting of SEQ ID NO:1, SEQ ID NO:3, and SEQ ID NO:5.
An isolated or purified protein fragment having antimicrobial activity, wherein said protein fragment is a polypeptide consisting of the sequence Z-2X-C-3X-C-(10–12) X-C-3X-C-3X-Z (SEQ ID NOS:
- 34–36)), wherein X is any amino acid residue other than cysteine, and C is cysteine, and Z is tyrosine or phenylalanine.
An isolated or purified protein fragment having antimicrobial activity, wherein said protein fragment is a polypeptide consisting of the sequence C-2X-C-3X-C-(10–12)X-C-3X-C-3X-C (SEQ ID NOS:
- 31–33), wherein X is any amino acid residue other than cysteine, and C is cysteine.
An isolated or purified protein fragment having antimicrobial activity, wherein said protein fragment is selected from the group consisting of:
- residues 29 to 73 of SEQ ID NO:1
- residues 74 to 116 of SEQ ID NO:1
- residues 117 to 185 of SEQ ID NO:1
- residues 186 to 248 of SEQ ID NO:1
- residues 29 to 73 of SEQ ID NO:3
- residues 74 to 116 of SEQ ID NO:3
- residues 117 to 185 of SEQ ID NO:3
- residues 186 to 248 of SEQ ID NO:3
- residues 33 to 75 of SEQ ID NO:5
- residues 76 to 144 of SEQ ID NO:5
- residues 145 to 210 of SEQ ID NO:5
- residues 34 to 80 of SEQ ID NO:7
- residues 81 to 140 of SEQ ID NO:7
- residues 33 to 79 of SEQ ID NO:8
- residues 80 to 119 of SEQ ID NO:8
- residues 120 to 161 of SEQ ID NO:8
- residues 32 to 91 of SEQ ID NO:21
- residues 25 to 84 of SEQ ID NO:22
- residues 29 to 94 of SEQ ID NO:24;
- and
- residues 31 to 85 of SEQ ID NO:25.
Dependencies are inferred from the claim text.