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AssigneeJapan Science and Technology Agency
InventorsHiroshi Sugiyama, Hirokazu Iida, Isao Saito, Takashi Saito
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It is intended to provide a method of producing a pyrrole-imidazole polyamide whereby a longer pyrrole-imidazole polyamide can be conveniently synthesized and a peptide (protein) can be easily transferred. According to this method, a pyrrole-imidazole polyamide having a carboxylate group which can be excised from a solid phase carrier at its end, makes it possible to directly transfer various functional groups and can exactly distinguish DNA sequences can be efficiently produced. A method of synthesizing a pyrrole-imidazole polyamide characterized by performing automatic synthesis by the solid phase Fmoc method with the used of a peptide synthesizer; a pyrrole-imidazole polyamide having a carboxyl group at its end obtained by this method; a pyrrole-imidazole polyamide having a DNA alkylation agent transferred into the carboxyl group at the end of the above-described pyrrole-imidazole polyamide; and a sequence-specific DNA alkylation method characterized by using the above compound.
A method of synthesizing a pyrrole-imidazole polyamide comprising performing a solid phase automatic peptide synthesis via a peptide synthesizer with a resin bound to a C-terminal of a β-alanine residue or a γ-aminobutyric acid residue by a Fmoc method with HATU[O-(7-azobenzotriazole-1-yl)-1,1,3,3-tetramethyluronium hexafluorophosphate]/DIEA(N,N-diisopropylethylamine).
A pyrrole-imidazole polyamide having β-alanine residue or γ-aminobutyric acid residue at its C-terminal end.
A FITC (fluorescein isothiocyanate)-pyrrole-imidazole polyamide conjugate having β-alanine residue or γ-aminobutyric acid residue at its C-terminal end.
Dependencies are inferred from the claim text.