Methods for introducing genes into mammalian subjects
AssigneeAntiCancer, Inc.
InventorsNorimitsu Saito, Ming Zhao
Life of this patent
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Abstract
Methods to obtain genetic modifications of cells in histoculture are described. Modification is assisted by treating the histoculture with collagenase prior to contacting the histoculture with the delivery vehicle for the desired gene. Hair follicles and other organized tissues can be modified in this was and then transplanted into intact recipients.
Claims14
A method to introduce a nucleic acid molecule into a mammalian subject which method comprises
- transplanting into the dermis of said subject at least one hair follicle that has been modified ex vivo to contain said nucleic acid molecule.
A method to introduce a nucleic acid molecule into a mammalian subject which method comprises transplanting into a corresponding tissue of said mammal a histocultured intact tissue that has been modified ex vivo to contain said nucleic acid molecule;
- wherein said histocultured intact tissue has been treated with collagenase prior to modifying said histocultured intact tissue with the nucleic acid.
A method of delivering a nucleic acid to a hair follicle which method comprises maintaining said hair follicle in histoculture and treating said histoculture with a nucleic acid;
- wherein said treating with a nucleic acid is preceded by the step of treating said histoculture with collagenase.
A method of delivering a nucleic acid to a an intact tissue which method comprises treating a histoculture of said intact tissue with said nucleic acid;
- wherein said treating with a nucleic acid is preceded by the step of treating said histoculture with collagenase.
Dependencies are inferred from the claim text.