Transport peptides such as C-terminal Erns peptide and analogues thereof
AssigneePepscan Systems B.V.
InventorJohannes Petrus M. Langedijk
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Abstract
The invention relates to peptides derived from or similar to the Ems protein of pestiviruses for type-specific diagnosis of infection, for eliciting antibiotic activity, and for transport of substances into a cell. For these purposes, the invention provides, among other things, an isolated, synthetic or recombinant protein or peptide module or functional equivalent thereof comprising an amino acid sequence having significant homology to, for example, an amino acid sequence of a peptide located from about amino acid position (a) 194 to about 220 in a pestiviral Ems protein (b) 59 to about 88 in an L3 loop of a cytotoxic RNase of a ribosome-inactivating protein, or (c) 187 to about 223 in a respiratory syncytial virus G-protein.
Claims23
An isolated peptide consisting of an amino acid sequence that is at least 70% identical to an amino acid sequence selected from the group of amino acid sequences consisting of
- from about amino acid position 194 to about amino acid position 220 in a pestiviral Ems protein RNase;
- from about amino acid position 59 to about amino acid position 88 in an L3 loop of a cytotoxic RNase of a ribosome-inactivating protein;
- and
- mixtures thereof.
An isolated peptide consisting of a reversed amino acid sequence to one of the group of amino acid sequences consisting of
- from about amino acid position 194 to about amino acid position 220 in a pestiviral Ems protein RNase;
- from about amino acid position 59 to about amino acid position 88 in an L3 loop of a cytotoxic RNase of a ribosome-inactivating protein;
- wherein, further, D-amino acids are used instead of L-amino acids.
A pharmaceutical composition comprising:
- a peptide with a length of up to 51 amino acid residues having heparin-binding activity, said peptide comprising an amino acid sequence selected from the group of amino acid sequences consisting of
- from about amino acid position 194 to about amino acid position 220 in a pestiviral Ems protein RNase;
- from about amino acid position 59 to about amino acid position 88 in an L3 loop of a cytotoxic RNase of a ribosome-inactivating protein;
- and
- a compound for delivery.
A method of eliciting antibiotic activity in a subject, said method comprising:
- administering to the subject a peptide consisting of an amino acid sequence selected from the group of amino acid sequences consisting of
- from about amino acid position 194 to about amino acid position 220 in a pestiviral Ems protein RNase;
- from about amino acid position 59 to about amino acid position 88 in an L3 loop of a cytotoxic RNase of a ribosome-inactivating protein;
- and
- mixtures of any thereof.
A method for translocating a compound through a cell's membrane, said method comprising:
- providing said compound with an isolated peptide consisting of an amino acid sequence selected from the group of amino acid sequences consisting of
- from about amino acid position 194 to about amino acid position 220 in a pestiviral Ems protein RNase;
- from about amino acid position 59 to about amino acid position 88 in an L3 loop of a cytotoxic RNase of a ribosome-inactivating protein;
- and
- contacting said compound and said isolated peptide with the cell.
An isolated peptide consisting of an amino acid sequence located at about amino acid position 194 to about amino acid position 220 in a pestiviral Ems protein RNase, wherein the isolated peptide has anti-bacterial activity for a gram-negative bacterium as determined by an anti-microbial assay comprising mixing the isolated peptide with a gram-negative bacterial suspension in a culture, incubating the culture overnight, and determining an absorbance of the culture.
An isolated peptide having anti-bacterial activity for a gram-negative bacterium as determined by an anti-microbial assay comprising mixing the isolated peptide with a gram-negative bacterial suspension in a culture, incubating the culture overnight, and determining an absorbance of the culture, the isolated peptide consisting of an amino acid sequence selected from the group of amino acid sequences consisting of
- from about amino acid position 194 to about amino acid position 220 in a pestiviral Ems protein RNase;
- from about amino acid position 59 to about amino acid position 88 in an L3 loop of a cytotoxic RN ase of a ribosome-inactivating protein;
- and
- mixtures of any thereof.
An isolated peptide consisting of a reversed amino acid sequence to one of the group of amino acid sequences consisting of
- from about amino acid position 194 to about amino acid position 220 in a pestiviral Ems protein RNase;
- and
- from about amino acid position 59 to about amino acid position 88 in an L3 loop of a cytotoxic RNase of a ribosome-inactivating protein;
- wherein, D-amino acids are used instead of L-amino acids.
Dependencies are inferred from the claim text.