Synthetic receptors, libraries and uses thereof

A method of preparing a synthetic receptor library with identifiers wherein said library comprises a plurality of different synthetic receptor members, and each synthetic receptor library member comprises a solid support having attached (1) a synthetic receptor comprising a template covalently linked to two or more oligomers, said template chosen from the group consisting of (a) monocyclic aliphatic hydrocarbons substituted with two or more groups to which oligomers are attached, (b) polycyclic aliphatic hydrocarbons substituted with two or more groups to which oligomers are attached, and (c) monocyclic heterocycles substituted with two or more groups to which oligomers are attached, said oligomers chosen independently from the group consisting of straight chained, cyclic and branched oligoamide, oligourea, oligourethane, oligosulfonamide, and peptide oligomers, said oligomers comprising three or more monomers and (2) one or more identifiers which define the synthetic receptor, the method comprising the steps of:

• a) reacting the solid supports in a reaction vessel with a template having a first template-protecting group and a second template-protecting group;
• b) removing said first template-protecting group;
• c) apportioning the solid supports with the attached template among a plurality of reaction vessels;
• d) reacting the template on a solid support in each reaction vessel with a monomer that will comprise said first oligomer, said monomer being protected with a first monomer-protecting group;
• e) reacting the solid supports in each reaction vessel with one of a plurality of first identifiers;
• f) pooling the solid supports;
• g) removing said second template-protecting group;
• h) apportioning the supports among a plurality of reaction vessels
• i) reacting the template on solid support in each reaction vessel with a monomer that will comprise said second oligomer, said monomer being protected with a second monomer-protecting group;
• j) reacting the solid supports in each reaction vessel with one of a plurality of second identifiers;
• k) pooling the solid supports;
• l) removing one of said first monomer-protecting group and said second monomer-protecting group;
• and
• m) repeating steps (c) through (f) plus (l) and (h) through (l) from at least one to twenty times for each oligomer of the synthetic receptor.

A method of preparing a synthetic receptor library with identifiers wherein said library comprises a plurality of different synthetic receptor members, and each synthetic receptor library member comprises a solid support having attached (1) a synthetic receptor comprising a template covalently linked to two or more oligomers, said template chosen from the group consisting of (a) monocyclic aliphatic hydrocarbons substituted with two or more groups to which oligomers are attached, (b) polycyclic aliphatic hydrocarbons substituted with two or more groups to which oligomers are attached, and (c) monocyclic heterocycles substituted with two or more groups to which oligomers are attached, said oligomers chosen independently from the group consisting of straight chained, cyclic and branched oligoamide, oligourea, oligourethane, oligosulfonamide, and peptide oligomers, said oligomers comprising three or more monomers and (2) one or more identifiers which define the synthetic receptor, the method comprising the steps of:

• a) apportioning the solid supports among a plurality of reaction vessels;
• b) reacting the solid supports in each reaction vessel with a monomer that will comprise a first oligomer, said monomer being protected with a first monomer-protecting group;
• c) reacting the solid supports in each reaction vessel with one of a plurality of first identifiers;
• d) pooling the solid supports;
• e) removing said first monomer-protecting group;
• f) apportioning the supports among a plurality of reaction vessels;
• g) reacting the solid supports in each reaction vessel with a monomer that will comprise a second oligomer, said monomer being protected with a second monomer-protecting group;
• h) reacting the pooled solid supports in each reaction vessel with one of a plurality of second identifiers;
• i) removing said second monomer-protecting group;
• j) repeating steps (a) through (e) and (f) through (i) from at least one to twenty times for each oligomer;
• and
• k) reacting the solid supports in each reaction vessel with said template.

A library comprising a plurality of distinct synthetic receptors covalently attached to a solid support and thereby to an identifier, wherein each synthetic receptor comprises a template covalently linked to two or more oligomers,

• (a) said template chosen from the group consisting of monocyclic aliphatic hydrocarbons substituted with two or more groups to which oligomers are attached, polycyclic aliphatic hydrocarbons substituted with two or more groups to which oligomers are attached, and monocyclic heterocycles substituted with two or more groups to which oligomers are attached;
• (b) said oligomers chosen independently from the group consisting of straight chained, cyclic and branched oligoamide, oligourea, oligourethane, oligosulfonamide, and peptide oligomers, said oligomers comprising two or more monomers, with the proviso that receptors containing only subunits of trimesic acid and 1,2 diaminocyclohexane are excluded;
• and
• (c) said identifier comprising a photosensitive linker and an electrophoric tag.

A method of preparing a synthetic receptor library with identifiers wherein said library comprises a plurality of different synthetic receptor members, and each synthetic receptor library member comprises a solid support having attached (1) a synthetic receptor comprising a template covalently linked to two or more oligomers, said template chosen from the group consisting of (a) monocyclic aliphatic hydrocarbons substituted with two or more groups to which oligomers are attached, (b) polycyclic aliphatic hydrocarbons substituted with two or more groups to which oligomers are attached, and (c) monocyclic heterocycles substituted with two or more groups to which oligomers are attached, said oligomers chosen independently from the group consisting of straight chained, cyclic and branched oligoamide, oligourea, oligourethane, oligosulfonamide, and peptide oligomers, said oligomers comprising three or more monomers and (2) one or more identifiers which define the synthetic receptor, the method comprising the steps of:

• a) reacting the solid supports in a reaction vessel with a template having a first template-protecting group and a second template-protecting group;
• b) apportioning the solid supports with the attached template among a plurality of reaction vessels;
• c) reacting the solid supports in each reaction vessel with one of a plurality of first identifiers;
• d) removing said first template-protecting group;
• e) reacting the template on a solid support in each reaction vessel with a monomer that will comprise said first oligomer, said monomer being protected with a first monomer-protecting group;
• f) pooling the solid supports;
• g) apportioning the supports among a plurality of reaction vessels;
• h) reacting the solid supports in each reaction vessel with one of a plurality of second identifiers;
• i) removing said second template-protecting group;
• j) reacting the template on solid support in each reaction vessel with a monomer that will comprise said second oligomer, said monomer being protected with a second monomer-protecting group;
• k) pooling the solid supports;
• and
• l) repeating steps (b) through (f) and (g) through (k) from at least one to twenty times for each oligomer of the synthetic receptor.