US 7,067,322 B2 · Fusion protein arrays on metal substrates for surface plasmon resonance imaging

Abstract

Disclosed are methods for making surface plasmon resonance-capable arrays wherein molecules, such as proteins or nucleic acids, or cells, are adhered to a metal substrate. The metal substrates are modified by depositing an ω-modified alkanethiol monolayer to the substrate and then contacting the ω-modified monolayer with a heterobifunctional linking compound. Biomolecules or cells can then be attached to the heterobifunctional linking compound. Also disclosed are arrays wherein glutathione-containing molecules are immobilized on the substrate and GST-containing molecules are then specifically immobilized onto the substrate, taking advantage of the affinity between glutathione and GST.

Claims13

Dependent claims

A method of immobilizing biomolecules or cells to a metal substrate comprising:

(a) depositing an amino-C8–C24-alkanethiol monolayer on a metal substrate;
(b) contacting the ω-modified monolayer of step (a) with a heterobifunctional compound comprising a first moiety reactive with the ω-modified alkanethiol monolayer and a second moiety comprising a protected thiol moiety, under conditions wherein the heterobifunctional compound binds to the ω-modified alkanethiol monolayer via the first moiety;
(c) deprotecting the second moiety of the heterobifunctional compound to yield an unprotected thiol moiety;
and then
(d) attaching a biomolecule or cell to the unprotected thiol moiety of step (c).

2 more elements

Dependencies are inferred from the claim text.

Facts

Application

10/099,424Filed 15 Mar 2002 · us provisional application

Priority

10 Jul 2001

Published

US 2003/0100127 A129 May 2003

Granted

27 Jun 2006

Term ends

Not estimatedThe source records don’t support an estimate.

Classification16 codes

C40B 40/06
Libraries containing only organic compounds: libraries containing nucleotides or polynucleotides, or derivatives thereof
B82Y 30/00
Nanotechnology for materials or surface science
C40B 30/10
Methods of screening libraries: by measuring physical properties, e.g. mass
C40B 50/18
Solid phase synthesis, i.e. wherein one or more library building blocks are bound to a solid support during library creation; Particular methods…:…

Fusion protein arrays on metal substrates for surface plasmon resonance imaging

Life of this patent

Fusion protein arrays on metal substrates for surface plasmon resonance imaging

Life of this patent

Abstract

Claims13