DNA and amino acid sequence of a tyrosine ammonia lyase enzyme from the bacterium Rhodobacter sphaeroides
AssigneeE. I. Du Pont de Nemours and Company
InventorsLixuan Huang, Zhixiong Xue
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Abstract
A novel tyrosine ammonia lyase enzyme was identified in the bacterium Rhodobacter sphaeroides. This enzyme has a higher activity for tyrosine than for phenylalanine and is useful for the production of para-hydroxycinnamic acid directly from tyrosine. The gene encoding this enzyme was cloned by direct amplification using the genomic DNA and was expressed in E. coli.
Claims9
An isolated nucleic acid molecule encoding a tyrosine ammonia lyase enzyme, selected from the group consisting of:
- a) an isolated nucleic acid molecule encoding the amino acid sequence as set forth in SEQ ID NO:3;
- b) an isolated nucleic acid molecule that hybridizes with (a) under the following hybridization conditions:
- 0.1×SSC, 0.1% SDS, 65° C. and washed with 2×SSC, 0.1% SDS followed by 0.1×SSC, 0.1% SDS;
- or an isolated nucleic acid molecule that is complementary to (a) or (b).
An isolated nucleic acid molecule as set forth in SEQ ID NO:2.
An isolated nucleic acid molecule comprising a first nucleotide sequence encoding a polypeptide of at least 526 amino acids that has at least 95% identity based on to Smith-Waterman method of alignment when compared to a polypeptide having the sequence as set forth in SEQ ID NO:3 or a second nucleotide sequence comprising the complement of the first nucleotide sequence, wherein said polpeptide has tyrosine ammonia lyase activity.
Dependencies are inferred from the claim text.