Materials and methods for in vitro production of bacteria
AssigneesPasteuria Bioscience, LLC, CDG Laboratories, Inc.
InventorsJohn F. Gerber, Thomas E. Hewlett, Kelly S. Smith, James H. White
Life of this patent
Estimating the term…
Abstract
The subject invention provides novel and advantageous methods for growing bacteria. The methods of the subject invention are particularly advantageous for growing parasitic bacteria in vitro, without the presence of host tissue. In one embodiment of the subject invention, Pasteuria endospores, such as those that infect the rootknot nematode Meloidogyne arenaria or other host nematodes, are grown in vitro under acidic conditions. The process of the subject invention is highly advantageous because Pasteuria can be grown in the absence of nematode tissue.
Claims12
A method for producing Pasteuria endospores in vitro, wherein said method comprises introducing bacteria Pasteuria into a growth medium that is free of sufficient nematode tissue to support bacterial growth, wherein the pH of said growth medium is less than about 6.0, and growing said bacteria Pasteuria.
Dependencies are inferred from the claim text.