Glucose dehydrogenase
OwnerKoji Sodeinventor
Life of this patent
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Abstract
Modified water-soluble glucose dehydrogenase having pyrrolo-quinoline quinone as a coenzyme are provided wherein at least one amino acid residue is replaced by another amino acid residue in a specific region. Modified water-soluble PQQGDHs of the present invention have improved thermal stability.
Claims6
An isolated protein comprising a variant of a glucose dehydrogenase consisting of the amino acid sequence of SEQ ID NO:1, wherein said variant glucose dehydrogenase has pyrrolo-quinoline quinone as a coenzyme, wherein said variant continues to have glucose dehydrogenase activity and said variant consists of at least one amino acid substitution selected from the group consisting of:
- (1) serine at position 207 substituted with an amino acid residue selected from the group consisting of lysine, asparagine, aspartate, histidine, methionine, leucine, and cysteine;
- (2) glutamine at position 185 substituted with lysine;
- (3) glutamate at position 186 substituted with lysine;
- (4) aspartate at position 396 substituted with lysine;
- and
- (5) alanine at position 397 substituted with aspartate.
Dependencies are inferred from the claim text.