Cystobacterineae host cells containing heterologous PKS genes for the synthesis of polykedtides
AssigneeKosan Biosciences, Inc.
InventorsBryan Julien, Leonard Katz, Chaitan Khosla
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Abstract
Recombinant host cells of the suborder Cystobacterineae, genus Stizmatella, containing recombinant expression vectors that encode heterologous epothilone polyketide synthase (PKS) genes and can produce epothilones at high levels are provided.
Claims4
A recombinant host cell of the genus Stigmatella containing a recombinant expression vector that comprises a heterologous epothilone polyketide synthase (PKS) gene cluster and produces a polyketide synthesized by an epothilone PKS encoded in said gene cluster wherein said host cell of produces epothilones C and D but not A and B.
A recombinant host cell of the suborder Cystobacterineae containing a recombinant expression vector that comprises a modified heterologous epothilone polyketide synthase (PKS) gene cluster and produces a polyketide synthesized by a PKS encoded in said gene cluster, wherein said host cell produces an epothilone derivative selected from the group consisting of 9-oxo-11-hydroxy-epothilone D;
- 9-hydroxy-11-oxo-epothilone D;
- and 9,11-dihydroxy-epothilone D.
Dependencies are inferred from the claim text.