Cell culture performance with betaine
AssigneeImmunex Corporation
InventorsBrian D. Follstad, Anne H. Potter
Life of this patent
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Abstract
This invention relates generally to the field of cell culture. More particularly, the invention relates to improving viability of recombinant cell cultures and the yields of secreted polypeptides therefrom by the addition of betaine to the tissue culture medium.
Claims25
A method comprising culturing a recombinantly
- engineered animal cell line in tissue culture medium, wherein the cell line is recombinantly engineered to express a polypeptide of interest, the medium has an effective amount of betaine and the tissue culture medium is not hyperosmotic, whereby cell survival and expression of said polypeptide of interest are improved relative to cells grown without betaine, wherein the cells are grown during a proliferative phase in the absence of betaine, and in the presence of betaine in an induction phase.
A cell culture comprising a recombinantly engineered animal cell in tissue culture medium, wherein the cell line is recombinantly engineered to express a protein of interest, the medium has an amount of betaine effective to improve cell survival and expression of said protein of interest and the tissue culture medium is not hyperosmotic, wherein the cells are grown during a proliferative phase in the absence of betaine, and in the presence of betaine in an induction phase.
Dependencies are inferred from the claim text.