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US 7,067,278 B1Granted
Nucleic acid encoding pigment protein from coral tissue
AssigneeThe University of Sydney
InventorsOve Hoech-Guldberg, Sophie Dove
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Abstract
Pigment protein derived from corals (PPCT), and polynucleotide molecules encoding the pigment protein are disclosed. The pigment protein is capable of emitting fluorescence upon irradiation by incident light, wherein maximal absorbance of the incident light is in the range of 320–600 nm, and maximal fluorescence emission is in the range of 300–700 nm. Uses of the pigment protein are also disclosed, especially as a tissue marker, fluorescent marker or general dyestuff.
Claims20
An isolated polynucleotide molecule comprising a nucleotide sequence selected from the group consisting of:
- SEQ ID NO:5, SEQ ID NO:6, a nucleotide sequence having at least 80% identity with SEQ ID NO:5, a nucleotide sequence having at least 80% identity with SEQ ID NO:6, a nucleotide sequence capable of hybridizing under high stringency conditions to the complementary strand of SEQ ID NO:5, and a nucleotide sequence capable of hybridizing under high stringency conditions to the complementary strand of SEQ ID NO:6, wherein said high stringency conditions include a washing step at 50° C. in a solution comprising 15 mM NaCl and 1.5 mM sodium citrate, and wherein said polynucleotide molecule encodes a pigment protein.
Dependencies are inferred from the claim text.