Peptide substrates of a proteolytic ADAM33 polypeptide and assays using the same
AssigneeSchering Corporation
InventorsRumin Zhang, Jun Zou, Feng X. Zhu, James P. Durkin, William T. Windsor, Shelby P. Umland
Life of this patent
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Abstract
The present invention discloses peptide substrates of a proteolytic ADAM33 polypeptide. The present invention also discloses methods of identifying additional substrates of a proteolytic ADAM33 polypeptide. Furthermore, the present invention discloses methods of identifying compounds that inhibit the proteolytic activity of a proteolytic ADAM33 polypeptide.
Claims13
An isolated peptide comprising the amino acid sequence EVAHQKLA (SEQ ID NO:
- 37) or Ac-YEVAHQKLVF-NH2 (SEQ ID NO:
- 13).
An isolated peptide comprising one conservative amino acid substitution in an amino acid sequence selected from the group consisting of EVAHQKLA (SEQ ID NO:
- 37) and Ac-YEVAHQKLVF-NH2 (SEQ ID NO:
- 13).
A method for identifying whether a test compound inhibits proteolytic activity of a proteolytic ADAM33 polypeptide comprising:
- a) contacting the proteolytic ADAM33 polypeptide with a substrate of the proteolytic ADAM33 polypeptide;
- b) measuring cleavage of the substrate;
- c) adding the test compound;
- d) measuring cleavage of the substrate in the presence of the test compound;
- wherein a decrease in cleavage of the substrate in the presence of the test compound indicates that the test compound inhibits the proteolytic activity of the proteolytic ADAM33 polypeptide.
An isolated peptide consisting of an amino acid sequence selected from the group consisting of:
- SGYEVAHQKLAFFA-OH (SEQ ID NO:
- 11);
- SGYEVAHQKLA-OH (SEQ ID NO:
- 9);
- Ac-YEVAHQKLVF-NH2 (SEQ ID NO:
- 13);
- YEVAHQKLAF-OH (SEQ ID NO:
- 7);
- YEVAHQKLA-OH (SEQ ID NO:
- 12);
- and
- EVAHQKLA-OH (SEQ ID NO:
- 37).
Dependencies are inferred from the claim text.