Thin membrane for measuring protease activity
AssigneeFuji Photo Film Co., Ltd.
InventorsRyoichi Nemori, Masayoshi Yamamoto, Kouki Nakamura, Yasunori Okada
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Abstract
A thin membrane for measuring protease activity, which is formed on a support and, which comprises one or more kinds of substances selected from the group consisting of a transferrin derivative and an albumin derivative and is crosslinked and/or substantially water-insoluble. The membrane enables selective measurement of protease activity of a particular class of protease such as matrix metalloproteinase 7.
Claims17
A thin membrane for measuring matrix metalloproteinase 7 (MMP-7) protease activity in a tissue slice or one or more cells, the membrane being formed on a support, and comprising at least one substance selected from the group consisting of a transferrin derivative and an albumin derivative, wherein said substance is crosslinked and/or substantially water-insoluble;
- wherein said transferrin derivative is obtained by:
- oxidation of a disulfide bond, cleavage of a disulfide bond with sulfite ion, or cleavage of a disulfide bond with a reducing agent, followed by S-alkylation;
- and wherein said albumin derivative is obtained by:
- oxidation of a disulfide bond, cleavage of a disulfide bond with sulfite ion, and cleavage of a disulfide bond with a reducing agent, followed by S-alkylation.
A method for detecting MMP-7 protease activity, which comprises the steps of:
- (1) bringing a tissue slice or one or more cells into contact with a thin membrane;
- and
- (2) detecting a trace of digestion formed on the thin membrane by activity of MMP-7 protease;
- wherein said thin membrane comprises at least one substance selected from the group consisting of a transferrin derivative and an albumin derivative, wherein said substance is crosslinked and/or substantially water-insoluble;
- wherein said transferrin derivative is obtained by:
- oxidation of a disulfide bond, cleavage of a disulfide bond with sulfite ion, or cleavage of a disulfide bond with a reducing agent, followed by S-alkylation;
- and wherein said albumin derivative is obtained by:
- oxidation of a disulfide bond, cleavage of a disulfide bond with sulfite ion, and cleavage of a disulfide bond with a reducing agent, followed by S-alkylation.
Dependencies are inferred from the claim text.