Method for detecting protein
AssigneeFuji Photo Film Co., Ltd.
InventorYukio Sudo
Life of this patent
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Abstract
An object of the present invention is to provide a method for detecting a protein using a nonradioactive label which is prevented to a smaller extent than in the case of the interaction of biotin/(strepto)avidin, and which achieves higher detection sensitivity than that of the detection system using DIG. The present invention provides a method for detecting a target substance, which comprises steps of: (A) allowing a target substance to come into contact with a protein labeled with a compound having a 6-membered ring, so as to carry out a binding reaction; and (B) detecting the protein labeled with the compound having a 6-membered ring, which was bound to the target substance, by using an antibody against the above compound having a 6-membered ring.
Claims9
A method for detecting a target substance, which comprises steps of:
- (A) allowing a target substance to come into contact with a protein that binds to said target substance and that is labeled with a compound having a 6-membered ring represented by the following formula (1), so as to carry out a binding reaction;
- and
- (B) detecting the protein labeled with the compound having a 6-membered ring, which was bound to the target substance, by using an antibody that specifically binds to the compound having a 6-membered ring, wherein the antibody is conjugated to a detectable label,
- wherein R1 and R2 may be identical or different, and may independently represent a hydrogen atom, or alkyl group containing 1 to 6 carbon atoms, and the dotted line represents an atomic group necessary for forming a 6-membered ring, and
- wherein the compound having a 6-membered ring represented by the formula (1) is a theophylline compound.
A method for detecting a target substance, which comprises steps of:
- (A) allowing a target substance to come into contact with a protein that binds to said target substance and that is labeled with a compound having a 6-membered ring represented by the following formula (1), so as to carry out a binding reaction;
- (B) reacting the protein labeled with the compound having a 6-membered ring, which is bound to the target substance, with an antibody that specifically binds to the compound having a 6-membered ring to form a target substance-protein-antibody complex;
- and
- (C) detecting the target substance-protein-antibody complex with a second antibody that specifically binds to the antibody in the target substance-protein-antibody complex, wherein the second antibody is conjugated to a detectable label,
- wherein R1 and R2 may be identical or different, and may independently represent a hydrogen atom, or alkyl group containing 1 to 6 carbon atoms, and the dotted line represents an atomic group necessary for forming a 6-membered ring, and
- wherein the compound having a 6-membered ring represented by the formula (1) is a theophylline compound.
Dependencies are inferred from the claim text.