US 7,067,267 B2 · Method of assaying pyrrole-containing biological compounds

Abstract

This invention relates to a method of assaying pyrrole-containing biological compounds and chemical compositions that can be used in the method. The method involves contacting a biological compound with one of: a) a bound or bindable derivatizing agent which forms a reaction product with the biological compound, followed by exposure to a detectable molecule which forms a complex with the reaction product; or b) a derivatizing agent which forms a reaction product with the biological compound, followed by exposure to a bound binding agent specific to the biological compound in the reaction product; or c) a binding agent specific to the biological compound, followed by exposure to a derivatizing agent which forms a reaction product with the biological compound, and determining the amount of bound biological compound. There is also provided a method of preparing an antigen.

Claims13

Dependent claims

A method for isolating pyrrole-containing biological compounds from a sample, the method comprising:

1) contacting the sample with a biotinylated Ehrlich's reagent wherein the biotinylated Ehrlich's reagent comprises a cleavable bond attached through the amine moiety of Ehrlich's reagent and the cleavable bond links the Ehrlich's reagent to biotin such that a reaction product is formed between the pyrrole-containing biological compound and the biotinylated Ehrlich's reagent;
2) contacting the reaction product with avidin or streptavidin attached to a solid support, such that the reaction product is bound to the solid support through biotin-avidin/streptavidin binding;
and
3) isolating the reaction product bound to the support by cleaving the cleavable bond of the Ehrlich's reagent.

1 more element

Dependencies are inferred from the claim text.

Method of assaying pyrrole-containing biological compounds

Life of this patent

Method of assaying pyrrole-containing biological compounds

Life of this patent

Abstract

Claims13