Sodium ion-driven chloride/bi-carbonate exchanger
AssigneesJCR Pharmaceuticals Co., Ltd., Susumu Seino
InventorsSusumu Seino, Hideki Yano, Changzheng Wang
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Abstract
The present invention discloses DNAs comprising nucleotide sequences set forth as SEQ ID NO:1 or NO:3 encoding Na+-driven Cl−/HCO3− exchanger, proteins comprising amino acid sequences set forth as SEQ ID NO:2 or NO:4, and their homologous proteins comprising an amino acid sequence having deletion, substitution, addition or insertion amino acids, which proteins, when expressed in a cell, functions as Na+-driven Cl−/HCO3− exchanger, and cells in which the proteins exogenously expressed.
Claims11
A cell in which a protein comprising the amino acid sequence set forth as SEQ IDS NO:2 or NO:4 in the Sequence Listing is expressed, wherein the cell is of a species different from the species of origin of the proteins.
A cell in which a protein is expressed comprising an amino acid sequence having deletion, substitution, addition or insertion of one to three amino acids relative to the amino acid sequence set forth as SEQ ID NO:2 or NO:4 in the Sequence Listing, which protein, when expressed in a cell, functions sodium ion-driven chloride/bicarbonate exchanger, and wherein the cell is of a species different from the species of origin of the protein consisting of the amino acid sequence set forth as SEQ ID NO:
- 2or SEQ ID NO:
- 4.
An isolated DNA comprising the nucleotide sequence set forth as SEQ ID NO:1 or NO:3 in the Sequence Listing.
An isolated DNA comprising a nucleotide sequence consisting of the nucleotides 67 through 3330 in the nucleotide sequence set forth as SEQ ID NO21 in the Sequence Listing.
An isolated DNA comprising a nucleotide sequence consisting of the nucleotides 83 through 3346 in the nucleotide sequence set forth as SEQ ID NO:3 in the Sequence Listing.
An isolated DNA comprising a nucleotide sequence having deletion, substitution, addition or insertion of one to ten nucleotides relative to a DNA comprising a nucleotide sequence consisting of the nucleotides 67 through 3330 in the nucleotide sequence set forth as SEQ ID NO:1 in the Sequence Listing, and encoding:
- (1) a protein comprising the amino acid sequence set forth as SEQ ID NO:2 in the Sequence Listing, or
- (2) a protein comprising an amino acid sequence having deletion, substitution, addition or insertion of one to three amino acids relative to the amino acid sequence set forth as SEQ ID NO:2 in the Sequence Listing, which protein, when expressed in a cell, functions as sodium ion-driven chloride/bicarbonate exchanger.
An isolated DNA comprising a nucleotide sequence having deletion, substitution, addition or insertion of one to ten nucleotides relative to a DNA comprising a nucleotide sequence consisting of the nucleotides 83 through 3346 in the nucleotide sequence set forth as SEQ ID NO:3 in the Sequence Listing, and encoding:
- (1) a protein comprising the amino acid sequence set forth as SEQ ID NO:4 in the Sequence Listing, or
- (2) a protein comprising an amino acid sequence having deletion, substitution, addition or insertion of one to three amino acids relative to the amino acid sequence set forth as SEQ ID NO:4 in the Sequence Listing, which protein, when expressed in a cell, functions as sodium ion-driven chloride/bicarbonate exchanger.
Dependencies are inferred from the claim text.