Generation of ion exchanger media
AssigneeGE Healthcare Bio-Sciences AB
InventorsJean-Luc Maloisel, Nicolas Thevenin
Life of this patent
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Abstract
The present invention relates to a method of generating a separation medium comprising mixed mode cation-exchanger ligands coupled to a base matrix, which method comprises to provide a scaffold comprising a functional group and exhibiting a cyclic core structure; derivatise the scaffold with a reagent comprising a reactive group coupled to a residue R by reacting the functional group of the scaffold with said reactive group; open the cyclic structure of the resulting derivative; and react the product with a base matrix comprising a reactive group. The scaffold presents at least two functionalities; one sulphur-comprising group for coupling to the base matrix and one group that can be transformed into an ionic group.
Claims11
A method of generating a separation medium including mixed mode cation-exchanger ligands coupled to a base matrix, which method comprises the steps of
- (a) providing at least one scaffold, said scaffold including a functional group (F) and having a cyclic core structure;
- (b) derivatizing each of the scaffold(s) with a reagent, which includes a reactive group (Z) coupled to a residue (R), by reacting the functional group of said scaffold with the reactive group of said reagent, while retaining the cyclic core structure of the scaffold;
- (c) opening up of the cyclic structure of the resulting derivative;
- and
- (d) reacting the opened product so obtained with a base matrix including a reactive group;
- wherein said scaffold presents at least two functionalities, one of which is a sulphur-comprising group for coupling to the reactive group of the base matrix and one of which is a group that can be transformed into an ionic group, said functionalities being present on the cyclic structure in adjacent positions, and wherein in step (c), opening up is provided by breaking the bond between two functionalities.
A method of generating a separation medium comprising mixed mode cation-exchanger ligands coupled to a base matrix, comprising
- (a) providing at least one scaffold derivative having a cyclic structure;
- (b) opening up of the cyclic structure of the derivative;
- and
- (c) reacting the opened product so obtained with a base matrix including a reactive group;
- wherein said scaffold derivative is of formula (III)
- wherein A, B and X are independently carbon atoms or any heteroatoms, and m is any integer between 0 and 4, and further wherein said functionalities are on the cyclic structure in adjacent positions, and wherein in step (c), opening up is provided by breaking the bond between said two adjacent positions, and wherein a F-Z-R is coupled to any one of A, B and X and wherein F is a functional group, Z is reactive group, and R is residue.
Dependencies are inferred from the claim text.