Method of preparing fertile transgenic corn plants by microprojectile bombardment
AssigneeDekalb Genetics Corporation
InventorsRonald C. Lundquist, David A. Walters, Julie A. Kirihara
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Abstract
A method of preparing fertile transgenic Zea mays (corn) plants which stably express DNA encoding a Bacillus thuringiensis endotoxin, so as to impart insect resistance to the fertile transgenic plants is taught. The method comprises bombarding regenerable corn cells with DNA-coated microprojectiles, wherein the DNA is adjusted to be more efficiently expressed in corn than the native B. thuringiensis sequence, and regenerating a fertile transgenic plants from the transformed cells.
Claims26
A process for producing a fertile transgenic Zea mays plant comprising the steps of (i) bombarding intact regenerable Zea mays cells with DNA-coated microprojectiles;
- wherein said DNA comprises a preselected DNA sequence encoding a Bacillus thuringiensis endotoxin, wherein the preselected DNA sequence is adjusted to be more efficiently expressed in Zea mays than the native B. thuringiensis DNA sequence encoding said endotoxin;
- (ii) identifying a population of transformed cells comprising said preselected DNA sequence;
- and (iii) regenerating a fertile transgenic plant therefrom, wherein said preselected DNA sequence is expressed so as to impart insect resistance to said transgenic plant and is heritable.
Dependencies are inferred from the claim text.