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US 7,064,245 B1Granted
Process for reconstructing a non-human mammalian embryo by nuclear transfer using a heat treated donor nucleus and preparaing a non-human mammal
AssigneeIstituto Zootecnico E Caseario per La Sardegna
InventorsPasqualino Loi, Pietro Cappai
Life of this patent
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Abstract
This invention relates to the generation of animals by the transfer of somatic cells which have been denatured preferably by heating, into enucleated metaphase II oocytes. This invention may be useful for the cloning of mammals including but not being limited to genetically selected and/or transgenic animals.
Claims19
A process for reconstructing an animal embryo of a species selected from the group consisting of mouse, cattle, sheep and goat, comprising:
- a. transferring into a recipient cell a diploid nucleus from a donor cell or the donor cell including said nucleus, said donor cell being a G1 or G0 cell from the same species and said recipient cell being an enucleated metaphase II oocyte of a non-primate mammalian species, wherein the chromatin within said nucleus is subjected to denaturing by heat-treating carried out at melting temperature of the transcriptional regulatory proteins before transferring the nucleus into the recipient cell;
- and
- b. further activating and culturing the recipient cell in vitro or in vivo.
Dependencies are inferred from the claim text.