Vectors for animal cells and utilization thereof
AssigneeKirin Beer Kabushiki Kaisha
InventorsElisa Misawa, Hiroaki Yajima, Keiji Kondo
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Abstract
Protein synthesis inhibitor resistance genes (typified by a cycloheximide resistance gene) are capable of imparting resistance to a protein synthesis inhibitor (typified by cycloheximide) to animal cells sensitive to the inhibitor. The genes have a sequence mutated by substitution in a gene encoding a ribosome-constituting protein derived from an animal. The genes may be placed in recombinant vectors, including expression vectors containing the gene together with a foreign protein structural gene.
Claims8
A protein synthesis inhibitor resistance gene that (A) imparts cycloheximide resistance to animal cells that are cyclohemide-sensitive (B) encodes an amino acid sequence comprising SEQ ID NO:1 or a 99% homologous sequence thereto, wherein said amino acid sequence contains an amino acid other than proline at the position corresponding to position 54 of SEQ ID NO:1.
Dependencies are inferred from the claim text.