Process for purifying antibody
AssigneeKyowa Hakko Kogyo Co., Ltd.
InventorsToyohide Shinkawa, Kazuhisa Uchida, Motoo Yamasaki, Emi Hosaka, Kenya Shitara
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Abstract
The present invention relates to a process for purifying an antibody having a desired property, which comprises using a substance having an affinity to a carbohydrate binding to the antibody; a medicament comprising, as an active ingredient, the antibody purified by the process; and a method for diagnosing or preventing various diseases, which comprises using a substance having an affinity to a carbohydrate binding to an antibody.
Claims8
A process for purifying an antibody composition having a desired property, which comprises:
- applying a solution containing the antibody composition to a column to which a lectin is immobilized to obtain a non-adsorbed fraction, said lectin being bound to a synthetic resin polymer;
- and
- recovering the antibody composition from the non-adsorbed fraction.
A process for purifying an antibody composition comprising an antibody having a carbohydrate structure to which bisecting N-acetylglucosamine is bound, which comprises:
- applying a solution containing the antibody composition to a column to which a wheat germ lectin or a Phaseolus vulgaris lectin E4 is immobilized to adsorb the antibody composition to the column, said lectin being bound to a synthetic resin polymer;
- eluting the antibody composition from the column with an eluent to obtain an eluted fraction;
- and
- recovering the antibody composition from the eluted fraction.
A process for purifying an antibody composition having a higher antibody-dependent cell-mediated cytotoxic activity than the antibody composition before purification, which comprises:
- applying a solution containing the antibody composition to a column to which a wheat germ lectin or a Phaseolus vulgaris lectin E4 is immobilized to adsorb the antibody composition to the column;
- eluting the antibody composition from the column with an eluent to obtain an eluted fraction;
- and
- recovering the antibody composition from the eluted fraction.
A process for purifying an antibody composition comprising an antibody having a carbohydrate structure to which fucose is not bound, which comprises:
- applying a solution containing the antibody composition to a column to which a Lens culinaris lectin is immobilized to obtain a non-adsorbed fraction, said lectin being bound to a synthetic resin polymer;
- and
- recovering the antibody composition from the non-adsorbed fraction.
A process for purifying an antibody composition having a higher antibody-dependent cell-mediated cytotoxic activity than the antibody composition before purification, which comprises:
- applying a solution containing the antibody composition to a column to which a Lens culinaris lectin is immobilized to obtain a non-adsorbed fraction;
- and
- recovering the antibody composition from the non-adsorbed fraction.
Dependencies are inferred from the claim text.