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US 7,064,190 B1Granted
Antigen-specific, activated T lymphocytes, detection and use
AssigneeRoche Diagnostics GmbH
InventorsJosef Endl, Peter Stahl, Winfried Albert, Guenther-Gerhard Jung, Dolores J. Schendel, Edgar Meinl, Klaus Dornmair
Life of this patent
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Abstract
The invention concerns autoreactive peptides, peptide-MHC complexes, T cell subpopulations that react thereto as well as diagnostic and therapeutic applications of these compounds.
Claims17
An isolated complex comprising:
- a peptide of glutamic acid decarboxylase which is selected from the group consisting of SEQ ID NO:
- 2, 3, 19–39 and a fragment thereof that has at least 6 contiguous amino acids of SEQ ID NO:
- 2, 3 or 19–39,
- which is bound to an allele of MHC Class II molecules DR3 or DR4 selected from the group consisting of DR B1 0301, DR B1 0401, DR B1 0402, DR B1 0404 and peptide-binding fragments thereof.
1 more element
An isolated complex comprising:
- a peptide of glutamic acid decarboxylase which is selected from the group consisting of SEQ ID NO:
- 2, 3, 19–39 and a fragment thereof that has at least 12 contiguous amino acids of SEQ ID NO:
- 2, 3 or 19–39,
- which is bound to an allele of MHC Class II molecules DR3 or DR4 selected from the group consisting of DR B1 0301, DR B1 0401, DR B1 0402, DR B1 0404 and peptide-binding fragments thereof.
1 more element
An isolated complex comprising:
- a peptide of glutamic acid decarboxylase which is selected from the group consisting of SEQ ID NO:
- 2, 3, 19–39 and a fragment thereof that has at least 6 contiguous amino acids of SEQ ID NO:
- 2, 3 or 19–39, wherein a reactive backbone and/or amino acid side group of said peptide has been derivatized by a chemical reaction,
- which is bound to an allele of MHC Class II molecules DR3 or DR4 selected from the group consisting of DR B1 0301, DR B1 0401, DR B1 0402, DR B1 0404 and peptide-binding fragments thereof.
1 more element
An isolated complex comprising:
- a peptide of glutamic acid decarboxylase which is selected from the group consisting of SEQ ID NO:
- 2, 3, 19–39 and a fragment thereof that has at least 12 contiguous amino acids of SEQ ID NO:
- 2, 3 or 19–39, wherein a reactive backbone and/or amino acid side group of said peptide has been derivatized by a chemical reaction,
- which is bound to an allele of MHC Class II molecules DR3 or DR4 selected from the group consisting of DR B1 0301, DR B1 0401, DR B1 0402, DR B1 0404 and peptide-binding fragments thereof.
1 more element
An isolated complex comprising:
- a peptide of glutamic acid decarboxylase which is selected from the group consisting of SEQ ID NO:
- 2, 3, 19–39 and a fragment thereof that has at least 6 contiguous amino acids of SEQ ID NO:
- 2, 3 or 19–39, wherein at least one amino acid of said peptide is replaced with a homolog of said amino acid selected from the group consisting of 4-hydroxyproline, 5-hydroxylysine, 3-methyl histidine, homoserine, ornithine, β-alanine and 4-aminobutyric acid,
- which is bound to an allele of MHC Class II molecules DR3 or DR4 selected from the group consisting of DR B1 0301, DR B1 0401, DR B1 0402, DR B1 0404 and peptide-binding fragments thereof.
1 more element
An isolated complex comprising:
- a peptide of glutamic acid decarboxylase which is selected from the group consisting of SEQ ID NO:
- 2, 3, 19–39 and a fragment thereof that has at least 12 contiguous amino acids of SEQ ID NO:
- 2, 3 or 19–39, wherein at least one amino acid of said peptide is replaced with a homolog of said amino acid selected from the group consisting of 4-hydroxyproline, 5-hydroxylysine, 3-methyl histidine, homoserine, ornithine, β-alanine and 4-aminobutyric acid,
- which is bound to an allele of MHC Class II molecules DR3 or DR4 selected from the group consisting of DR B1 0301, DR B1 0401, DR B1 0402, DR B1 0404 and peptide-binding fragments thereof.
1 more element
Dependencies are inferred from the claim text.