Chimeric G protein coupled receptors
AssigneeSRI International
InventorsGeetha Shankar, Jason N. Munning, Juliet V. Spencer
Life of this patent
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Abstract
The present invention provides chimeric Edg receptors, useful as components of an assay system. The present invention also provide methods of screening for compounds that bind a chimeric Edg receptor, for example, by contacting a chimeric Edg receptor with a compound and detecting a change in chimeric Edg receptor-mediated activity such as calcium mobilization.
Claims17
A chimeric Edg receptor selected from the group consisting of Edg 1/3(i3ct), Edg 1/3(i2i3ct) Edg 5/13(i3ct) comprising a portion of a first Edg receptor and a portion of a second Edg receptor, wherein the chimeric Edg receptor comprises:
- (a) a non-contiguous replacement of at least one intracellular domain strand of a first Edg receptor;
- (b) with a corresponding strand from a second Edg receptor.
A chimeric Edg receptor comprising:
- a) an extracellular domain of a first Edg receptor;
- b) a transmembrane domain of the first Edg receptor, wherein the transmembrane domain is operably linked to the extracellular domain;
- and
- c) a chimeric intracellular domain comprising a third intracellular loop and a carboxy teîminal strand of a second Edg receptor, wherein the chimeric intracellular domain is operably linked to the transmembrane domain.
A nucleic acid encoding a chimeric Edg receptor selected from the group consisting of Edg 1/3(i3ct), Edg 1/3(i2i3ct) and Edg 5/3(i3ct) comprising a portion of a first Edg receptor and a portion of a second Edg receptor, wherein the chimeric Edg receptor comprises:
- (a) a non-contiguous replacement of at least one intracellular domain strand of a first Edg receptor;
- (b) with a corresponding strand from a second Edg receptor.
Dependencies are inferred from the claim text.