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US 7,063,962 B2Granted
DNA sequences for regulating transcription
AssigneeNovozymes A/S
InventorJan Lehmbeck
Life of this patent
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Abstract
The invention relates to a DNA sequences for regulating transcription of a structural gene encoding a polypeptide in a eukaryotic host cell comprising (a) a first DNA sequence to which RNA polymerase binds which DNA sequence comprises a mRNA initiation site; and further (b) one or more DNA sequence(s) to which RNA polymerase binds with or without a mRNA initiation site. The invention also relates to a DNA construct and an expression vector and a host cell comprising the DNA sequence of the invention.
Claims23
A DNA sequence for regulating transcription of a structural gene encoding a polypeptide in a eukaryotic host cell comprising:
- (a) an NA2-tpi promoter comprising SEQ ID NO:1, and
- (b) one or more additional promoters with or without an mRNA initiation site, wherein the NA2-tpi promoter and the one or more additional promoters are operably linked to the structural gene.
Dependencies are inferred from the claim text.