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US 7,063,955 B2Granted
Method for production of asymmetric carotenoids
AssigneeE. I. Du Pont de Nemours and Company
InventorsQiong Cheng, Luan Tao
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Abstract
Genes have been isolated from Rhodococcus and Deinococcus which encode a specific lycopene β-cyclase capable of converting acyclic carotenoids with at least one ψ-end group to the corresponding asymmetric carotenoid containing a single β-ionone ring end group. The genes are new. Transformed host cells expressing the present genes and methods for the bio-conversion of acylic carotenoid substrates to corresponding asymmetric carotenoid are also provided.
Claims1
A method for the production of γ carotene or 4-keto-γ-carotene comprising:
- a) providing a host cell which produces lycopene or neurosporene wherein said host cell expresses a lycopene β-cyclase that converts lycopene or neurosporene into γ-carotene or 4-keto-γ-carotene and is encoded by a nucleic acid molecule selected from the group consisting of:
- (i) an isolated nucleic acid molecule encoding the amino acid sequence of SEQ ID NO:2;
- and;
- (ii) an isolated nucleic acid molecule that hybridizes with (i) under the following hybridization conditions:
- 0.1×SSC, 0.1% SDS, 65° C. and washed with 2×SSC, 0.1% SDS followed by 0.1×SSC, 0.1% SDS;
- b) growing the host cell of (a) under conditions whereby γ-carotene or 4-keto-γ-carotene are produced;
- and
- c) Optionally recovering the γ-carotene or 4-keto-γ-carotene.
5 more elements
Dependencies are inferred from the claim text.