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US 7,063,953 B2Granted
Human voltage gated sodium channel β1A subunit and methods of use
AssigneeOrtho-McNeil Pharmaceutical, Inc.
InventorsNing Qin, Ellen Codd, Michael D'Andrea
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Abstract
DNAs encoding human voltage gated sodium channel β1A subunit have been cloned and characterized. The recombinant protein is capable of forming biologically active protein. The cDNA's have been expressed in recombinant host cells that produce active recombinant protein. The recombinant protein is also purified from the recombinant host cells. In addition, the recombinant host cells are utilized to establish a method for identifying modulators of the receptor activity, and receptor modulators are identified.
Claims3
A method of screening for a modulator of sodium channel activity comprising:
- (a) providing a cell that co-expresses a protein encoded by SEQ ID NO:12 and a sodium channel α subunit protein, wherein the cell elicits a sodium ion flux;
- (b) contacting the cell with a putative β1A modulating compound;
- (c) measuring a change upon the cell that alters the sodium ion flux;
- and
- (d) comparing said change to a base value observed in an otherwise identical cell that does not express said encoded protein.
2 more elements
Dependencies are inferred from the claim text.